Single-pot, solid-phase-enhanced sample preparation for proteomics experiments | Nature Protocols
Solvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Processes | Free Full-Text | Evaluation of Filter, Paramagnetic, and STAGETips Aided Workflows for Proteome Profiling of Symbiodiniaceae Dinoflagellate
Solvent Precipitation SP3 (SP4) Enhances Recovery for Proteomics Sample Preparation without Magnetic Beads | Analytical Chemistry
Implementing solid-phase-enhanced sample preparation for Co-Immunoprecipitation with Mass Spectrometry for C. elegans | bioRxiv
Reproducible protein and peptide cleanup for mass spectrometry | Cytiva
Extending the Compatibility of the SP3 Paramagnetic Bead Processing Approach for Proteomics | Journal of Proteome Research
SP3 meets iST – protein sample preparation for dirty and difficult sample matrices simplified
Ultrasensitive proteome analysis using paramagnetic bead technology | Molecular Systems Biology
Improving Proteome Coverage for Small Sample Amounts: An Advanced Method for Proteomics Approaches with Low Bacterial Cell Numbers - Blankenburg - 2019 - PROTEOMICS - Wiley Online Library
SP3-based protein sample preparation simplified | PreOmics
Cysteine enrichment with single-pot, solid-phase-enhanced sample... | Download Scientific Diagram
Solvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads | bioRxiv
Single-pot, solid-phase-enhanced sample preparation for proteomics experiments | Nature Protocols
Rahul Samant on X: "As promised, a 🧵 to convince you to consider SP4 for your next #proteomics sample prep! TL;DR: Cell lysate -> pure peptides ready for LC-MS with 20-30 min
Rahul Samant on X: "Deep proteome profiling—TMTsixplex, Lys-C+trypsin digestion, followed by off-line peptide fractionation into 62 fractions—showed almost identical results for SP3, SP4 (bead-free), and SP4 (+glass beads), WITH ONE NOTABLE EXCEPTION…